RABIES


INTRODUCTION
Rabies is an infectious disease of animals caused by a bullet-shaped, enveloped RNA virus, 180 x 75 nm. Man is occasionally infected, and once infection is established in the CNS, the outcome is almost invariably fatal.


HUMAN RABIES
Is acquired from virus in saliva entering a bite wound caused by an infected animal, usually a rabid dog. The severity of the bite determines the risk of infection. The disease does not usually spread from man to man.

Incubation
After inoculation, the virus enters small nerve endings at the site of the bite. The virus slowly travels up the nerve to reach the CNS where it replicates and then travels down nerves to the salivary glands where there is further replication. The time it takes to do this depends upon the length of the nerve - a bite on the foot will have a very much longer incubation period than a bite on the face. The incubation period may last from two weeks to six months. Very often the primary wound is healed and forgotten by the time of clinical presentation.

Clinical Presentation

A) Furious Rabies
When the virus reaches the CNS the patient presents with headache, fever, irritability, restlessness and anxiety. This may progress to muscle pains, salivation and vomiting. After a few days to a week the patient may experience a stage of excitement and be wracked with painful muscle spasms, triggered sometimes by swallowing of saliva or water. Hence they drool and learn to fear water (* Hydrophobia). The patients are also excessively sensitive to air blown on the face. The stage of excitement lasts only a few days before the patient lapses into coma and death.
Once clinical disease manifests, there is a rapid, relentless progression to invariable death, despite all treatment.

B) Dumb Rabies
Starts in the same way, but instead of progressing into excitement, the subject retreats steadily and quietly downhill, with some paralysis, to death. Rabies diagnosis may easily be missed.


ANIMAL RABIES
Very similar picture to human rabies. In the stage of excitement the animal may bite vigorously and viciously at anything: sticks, stones, grass, other animals and humans, without provocation.

Wild animals may be abnormally tame or appear sick - beware of approaching or picking up such an animal ("dumb rabies").

Epidemiology

1. The disease is endemic in wild animals in most parts of the world although some countries (UK, Australia) are rabies free through vigorous control. The wild animal cycle constitutes the natural reservoir.

2. Wild animals may bite and infect domestic animals (cattle, horses, pigs, dogs and cats) which in turn may infect man. Occasionally wild animals may infect man directly.

3. In recent decades, a separate form of dog rabies (spread from dog to dog) has been recognised as spreading from West Africa eastwards and southwards in Africa. Via Mozambique, it reached Natal Kwazulu in the late 1970's and early 80's. Semi-wild dogs in Natal have formed the highest endemic rabies reservoir and source for human cases in the whole of the RSA.


ANIMAL RESERVOIR

Note: As in man, an infected animal becomes sick and dies. There is no substantial evidence of a true carrier status in apparently well animals, except perhaps in bats.


DIAGNOSIS
By assessment of:
1.Bite
Geographical area, type of animal, severity and site of bite.

2. Animal

Live - observe in cage:
If survives > 8 days, then NOT rabies.
Dead - brain sent to Onderstepoort
- Negri bodies
- IFA
- virus isolation

3. Man

Live - difficult diagnosis
- clinical picture, skin biopsy, corneal impression (antibodies only appear very late)
Dead - brain sent to Onderstepoort
"Negri bodies" in cytoplasm of brain cells;
immunofluorescence virus isolation


TREATMENT


RABIES VACCINE
A good but expensive killed virus vaccine (Human Diploid Cell Vaccine, HDCV) grown in human fibroblasts is available for safe use in man.
The unusually long incubation period of the virus permits the effective use of active immunisation with vaccine post-exposure. When used, vaccine has dramatically cut the rabies death rate. Supplied free by the State through district surgeons in South Africa.
(Older killed virus vaccines, made from infected neural tissues, were poorly immunogenic and had allergic encephalitic side effects, but are still used in developing countries.)

Prophylaxis
High-risk persons, eg. veterinarians, may be immunised before exposure, and then merely require one or two booster doses if they should be exposed to rabies.

Animal Vaccines
A range of live and killed virus vaccines are available for domestic animals (farm animals, cats and dogs).

Experimental vaccination of wildlife by using recombinant vaccinia vaccine (live) in bait for foxes in Europe and North America has been quite promising.


CONTROL OF RABIES (Government Department of Veterinary Services)
1. Education
2. Vaccination of dogs, cats and farm animals.
3. Notification
- animals (district vet officer, police, magistrate) - human (district surgeon)


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These notes were prepared by Anthony Keen
for Virology Lectures to 3rd Year Medical Students
in the Department of Medical Microbiology , University of Cape Town.

Illustrations and layout by Linda Stannard, 1995 ©