This is a partially completed html page in a set of molecular biology techniques, recipes and protocols pages. Please read the disclaimer and the introductory pages. Your submissions and comments are always welcome. The pages have been assembled by Brendan Babb at the department of Medical Microbiology, University of Cape Town.

BBHelp: Molecular Biology Help

On-line windows help for molecular biologists, including protocols, quick protocol guides, recipes and various useful appendices. This is a partial conversion of a windows help file (bbhlp087.zip). Due to time constraints I will not be able to complete the conversion to HTML format in the near future.

How to contribute

How to use BBHelp

Protocols

I. Bacterial methods: DNA isolation, transformation, RNA isolation...

II. DNA related protocols: Restriction, ligation, Southern blots, ...

III. RNA related protocols: Northern blots, primer extension, ...

IV. Protein related protocols: SDS-PAGE gels, Western blots, ...

V. Polymerase Chain Reaction (PCR): Descriptions and discussion of PCR techniques,

List of all recipes

Keywords: List of all recipes; recipe

Recipes

Please look up entries in alphabetical order of their full names. Where not otherwise stated, storage is at room temperature

A

acrylamide gel mix

Acrylamide stock

Ammonium acetate

Ammonium persulphate

B

Bal31 dilution/storage buffer

Bal31 reaction buffer

Blocking buffer stock solution, Westerns

Blocking buffer, Westerns

Blot buffer, Westerns

BSA (bovine serum albumin)

C

Calcium chloride, CaCl2

Cell Suspension buffer

Chloroform-isoamyl alcohol mix (24:1)

Church hyb buffer

Church prehyb buffer CHB

CTAB/NaCl (hexadecyltrimethyl ammonium bromide)

D

Denaturation solution 2 (0.4M NaOH/1M NaCl)

Destain, SDS-PAGE

Dithiothreitol, DTT

DNA digestion: core reaction buffers

DNA digestion: enzyme dilution buffer

E

Ethidium bromide, EtBr

ExoIII reaction buffer

F

G

Gel mixes, SDS-PAGE

H

Hybridization solution (Zappe)

I

IPTG, Isopropyl-á-D-thio-galactopyranoside

Isopropanol, salt-saturated

J

K

Klenow buffer (10x)

L

Laural sulphate, see sodium dodecyl sulphate

Ligation dilution buffer

Ligation reaction buffer

Luria broth, LB

Lysing solution

M

Magnesium chloride, MgCl2

Magnesium sulphate, MgSO4

Minimal medium, E. coli

Minimal medium 5x salts, E. coli

Mungbean nuclease dilution/storage buffer

Mungbean nuclease reaction buffer

N

O

P

PB (phosphate buffer)

Phenol Chloroform mix

Phenol (buffered pH 7.6, 10mM Tris)

Potassium chloride

Potassium salts

Q

R

Resolving buffer, SDS-PAGE

S

Salt, see sodium chloride

Sample buffer, SDS-PAGE

SDS, see sodium dodecyl sulphate

Sma I reaction buffer

Sodium acetate, NaAc

Sodium chloride, NaCl

Sodium dodecyl sulphate, SDS

Sodium hydroxide, NaOH

Sodium perchlorate, NaClO4

Solution III, plasmid preps

Solution II, plasmid preps

Solution I, plasmid preps

SSC (salt sodium citrate) 20x

ST1 Southern transfer solution 1

ST2 Southern transfer solution 2

ST3 Southern transfer solution 3

Stacking buffer, SDS-PAGE

Stain, SDS-PAGE

T

TAE buffer,Tris-acetate-EDTA

Tank buffer, SDS-PAGE

Taurine buffer (TTE)

TE buffer, Tris-EDTA buffer

Tracking dye (IV)

Tracking dye (Coyne)

U

V

W

Wash buffer A (WBA)

Wash buffer B (WBB)

Wash buffer C (WBC)

X

X-gal, 5-bromo-4-chloro-3-indolyl-á-D-galactoside

Y

YT 2x, yeast tryptone medium

Z