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IMAGING, CALORIMETRY & SPECTROPHOTOMETRY CORE FACILITY
The Core Facility is an inter-departmental facility situated in Chemical Pathology, which performs chemical imaging, electronic autoradiography, isothermal titration calorimetry & UV/VIS spectrophotometry using current technology.
| Alpha-Innotech Chemi-Imager |
- Digital imaging of visible, UV, fluorescent and chemiluminescent samples.
- e.g. protein gels, autorads, film, plates, fluorescent gels, colorimetric blots and membranes, TLCs , etc.
- Reflective and transilluminated visible (white) & UV light sources.
- Colour filters to correspond with your sample staining.
- Software for image enhancement and analysis.
- Acquisition and analysis can be done by the facility operator or the user.
- Please contact me here to book the Chemi-Imager if you require it for more than 30 min and I will add your booking to the calendar below.
Click the Week or Agenda tab for a more detailed view.
New User Form & Rates
New User Form & Rates |
| Cyclone Storage Phosphor System (Phosphor-Imager) |
- Electronic autoradiography system which measures radioactivity in any flat sample e.g. protein gels, blots, membranes, TLCs.
- Comprised of storage phosphor screens to expose your sample, a laser scanning device to read the resulting latent image, and image analysis software for quantifying the radioactivity.
- Any radionuclide can be read e.g. 14C, 35S, 32P, 3H.
For 3H there are special phosphor screens available.
- Capable of producing very high resolution images up to 600dpi.
- The Cyclone scanner is operated by skilled personnel only.
Image analysis can be done by the user.
- Please contact me here to book the Phosphor Imager.
Request Form & Rates |
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| ITC200 Isothermal Titration Calorimeter |
- Isothermal Titration Calorimetry (ITC) is a powerful analytical tool which measures the binding affinity and thermodynamics between any two biomolecules.
- ITC directly measures the heat released or absorbed during molecular binding.
- Measurement of this heat enables direct accurate determination of these thermodynamic parameters in a single experiment: binding affinity (KD), stoichiometry (n), enthalpy (ΔH) and entropy (ΔS)
- Wide range of applications including characterization of molecular interactions with small molecules, proteins, antibodies, nucleic acids, lipids, metal ions, etc., as well as enzyme kinetics & inhibitor studies.
- Small volumes: 200 μl of sample per experiment.
- The ITC200 is operated by skilled personnel only.
Analysis can be done by the user.
- Visit the Microcal website for more information about ITC.
- Please contact me here to book the ITC200 and I will add your booking to the calendar below. Click the Week or Agenda tab for a more detailed view.
Request Form & Rates
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| Agilent 8453 Diode Array UV/VIS Spectrophotometer |
- UV-Vis spectral acquisition and analysis in less than 1 second.
- Absorbance & time-based kinetic modes.
- Powerful acquisition & data analysis software.
- Acquisition and analysis can be done by the facility operator or the user.
- Please contact me here to book the spectrophotometer.
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The Core Facility is housed in Room 6.47 on the 6th level of the Falmouth Building on the Health Sciences campus. It is operated on a cost-recovery basis and the income generated is used for the purchase of reagents, maintenance, repairs and upgrading and expansion of the existing facilities to meet the demands of its users.
| Contact us |
Room 6.47
Falmouth Building
UCT Medical School
Observatory 7925
Cape Town
South Africa. |
Tel: +27-21-4066104
Fax: +27-21-4488150
Email:
dave.woolley@uct.ac.za |
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